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IGF-I itself is a small protein consisting of 70 amino acids, with a molecular weight of 7.65 kDa, and the gene is located at chromosome 12q23 (10)

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The microbiota is involved in numerous physiological processes, including digestion, immune modulation, and the production of essential vitamins and short-chain fatty acids (SCFAs) like butyrate, which contribute to intestinal barrier integrity and anti-inflammatory effects (Ahmed et al., 2022)