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For both propagation as well as the treatment, cells were maintained in an incubator (Heraeus, GmbH, Germany) at 37C

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Many investigations have shown that crude black seed or its active components have various biological activities, including anti-cancer, anti-oxytocic, anti-tussive, anti-pain, antipyretic, analgesic, and antioxidant activities []

15 Other 6 fatty acids such as arachidonate can cure the scaly skin symptoms of EFA deficiency, although evidence indicates their retro-conversion to linoleate for functionality in the epidermis