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3.4.4 Guidance for FBS alternatives selection Selecting an appropriate alternative to FBS depends on several factors including cell type, research application, regulatory needs, ethical considerations, and budget constraints (van der Valk, 2022

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All cells were placed at 37C with 5% CO 2 in 154CF medium (Gibco, live cell imaging) or CnT-PR medium (CELLnTEC, immunofluorescence)