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The homozygosity of T-DNA insertion mutants was verified by PCR using T-DNA specific and target specific primers (Supplementary Table S2)

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In wheat leaves, -ECS activities are strongly associated with chloroplasts (Noctor et al., 2002) and subcellular localization studies of the Arabidopsis protein have convincinglydemonstrated that the enzyme is restricted to plastids (Wachter et al., 2005)

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Compounded semaglutide with b12 has become increasingly common in weight loss clinics and compounding pharmacies across the country