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RODEO has also been used for the mining of RiPPs in the sactipeptide 68 and thiopeptide families

5) (Alary et al., 2003)

(Created with biorender.com) Thus, it is questionable whether this mechanism can be applied to other cell types, particularly hepatocytes with their complex, multi-layered antioxidant systems

Briefly, frozen tissue was powdered with a nitrogen-cooled mortar and pestle, and 50-70mg of tissue were homogenized in the absence (for GSH determination) or presence (for GSSG determination) of 1-methyl-2-vinylpyridinium trifluoromethanesulfonate to scavenge reduced thiols

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