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The His 6 -tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid M pro (see Figure S1A in supplemental material) and C300S M pro were further purified by SEC using a HiLoad Superdex 200 pg column (GE Healthcare) in 20 mM Tris, pH 7.5, 150 mM NaCl, and 2 mM DTT

Materials and methods Plant material and treatments The cucumber variety used in this study was Chunqiuwang NO

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