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Twenty-four hours later, cells at approximately 80% confluence were co-transfected with pCS2+8-NCBP1-Halo, pCS2+8-Cand1-Halo or pCS2+8-IPO5-Halo, actin(K118TAG) and MmPylRS RNA synthetase (ratio, 1:1:0.2

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The RNA was subjected to cDNA synthesis with Fastking One Step RT-PCR Kit (TIANGEN Biotech, Hainan, China) following the instructions of the reagent manufacturer

Pathophysiology of mucus in critical illness Mucus dysfunction in critical illness can arise from both the primary disease and the treatments administered in ICU (Table 1)