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3.7 Others In addition to the harmful products generated from the nutrient and energy metabolism pathways discussed above (such as lactate, kynurenine, and adenosine), other toxic metabolites produced from some tumor-specific activities in the TME can also have a negative effect on infiltrating lymphocytes

However, looking at the results for enzyme expression and activity (Figure 4A,B and Figure 5A,B), it can be seen here that the addition of DMSO results in the production of sulfane sulfur-containing compounds (expression and activity of CTH in serum-free medium increases, Figure 4B and Figure 5B), and there is an increase in the expression level of TST (Figure 4A,B), possibly enabling the activity of this enzyme to be maintained at control levels (no statistically significant change in TST activity, Figure 5A,B)

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The mixture was then incubated at ambient temperature (22 C) for 30 min to separate the organic and aqueous phases

Discontinue and reassess if shedding seems disproportionate to expected adjustment
