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In HFD-fed KK-Ay mice, L-carnitine administration inhibited Srebp1c mRNA overexpression while increasing -hydroxybutyrate levels, suggesting concurrent lipogenesis suppression and -oxidation activation (33) provided mechanistic depth by demonstrating that carnitine orotate complex increases carnitine acetyltransferase (CrAT) and -oxidation-related proteins (CPT1, PPAR, UCP2) while inhibiting SREBP1c nuclear translocation and downstream target gene expression ( Hmgcr, Srebp2, Pcsk9, Ldlr ) in HFD-fed mice

Longitudinal studies have shown elevated inflammatory markers as a predictor of increased incidence of mobility limitation [12] or the risk of muscle strength loss [13]

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The synthesis conditions used were as follows: an initial cycle at 50 C for 2 min, one cycle of polymerase activation at 95 C for 10 min, followed by 40 cycles with a denaturation temperature at 95 C for 15 s, an alignment of 60 C by 60 s and an extension at 72 C by 45 s