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Description
Derivation of blood-brain barrier endothelial cells from human pluripotent stem cells
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The most significant results came from the in vivo animal studies
(2) Sterilization: aseptic processing, though essential, adds significant cost
After discarding the medium, 100 l 10% CCK-8 solution was added to each well, followed by incubation at 37C for 1 h
Plasmid construction All primers used for DNA construction are listed in Supplementary Table 1
