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Since most of the physiological properties of the GL are determined by the degree of GAG sulfation, ways to optimise inorganic sulfate, the rate-limiting substrate for sulfation, should be the primary focus of treatment

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We have summarized the above content and created a summary table for reference (Table 2

Cell membrane permeabilization was achieved using 0.1% Triton X-100 in PBS for 10 min, followed by immunological blocking with 1% BSA in PBS for 60 min