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It relies on intense acid treatment to terminate enzymatic reactions at predetermined intervals, followed by protein precipitation steps that complicate implementation in microplate-based high-throughput systems [16]

Thermal processes such as boiling or prolonged heating can result in up to 50% reduction in total phenolics, including luteolin, whereas controlled enzymatic or fermentative processing can enhance the aglycone content, thereby improving intestinal absorption and bioavailability ( 3.3 Pharmacokinetics and bioavailability A clear understanding of luteolins pharmacokinetic behavior is essential for translating its preclinical promise into therapeutic applications in reproductive disorders