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The duration of semaglutide use varies, but many protocols continue for 40 to 68 weeks total

The tissue samples were homogenised in phosphate-buffered saline (PBS, pH 7.4) containing 0.1 mM butylated hydroxytoluene (BHT) (TissueRuptor, Qiagen, Valencia, CA, USA) and sonicated for 30 s in an ultrasonic ice bath (Branson, USA)

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