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A power calculation indicated that with the variation we saw in daily food intake, a group size of 57 would give us a high probability of correctly selecting compounds if we aimed for 30% reduction in the food intake over 24 h

These primary antibodies were incubated with brain sections in blocking buffer at 4 C overnight

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[34] Tang L, Jin T, Zeng X, et al

Several observational studies using similar formulations and instillation protocols ranging from weekly to monthly to PRN and follow-up intervals of a few months to several years reported efficacy rates of 25 to 90%

Severe hyperglycaemia may induce glucotoxicity in selective neurons involved in cognitive processing, with hippocampal neurons having been identified as particularly vulnerable to hyperglycaemia, and with proposed mechanisms including mitochondrial dysfunction and inflammatory responses [35]