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Guzik TJ, West NE, Black E, McDonald D, Ratnatunga C, Pillai R, Channon KM (2000) Vascular superoxide production by NAD(P)H oxidase: association with endothelial dysfunction and clinical risk factors

Seyoum AB

RT-qPCR was performed on the StepOnePlus Real-Time PCR Systems (Thermo, Waltham, MA, USA)

After incubation with horseradish peroxidase-conjugated secondary antibody (Proteintech, USA) for 2 h at room temperature, the signals were visualized using an ECL kit (Millipore, USA)

doi: 10.1371/journal.pone.0035739

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