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Plasma metabolomics for amino acids and pyruvate Amino acids were extracted from 20 L of plasma with 200 L of methanol containing Try-d8 (1600ng), Phe-d8 (160ng), Tyr-d4 (160ng), Leu-d3 (320ng), Ile-13C6,15N (160ng), Met-d3 (160ng), Val-d8 (400ng), Glu-d3 (2400ng), Thr-13C4 (320ng), Ser-d3 (400ng), Asp-d3 (3200ng), Ala-d4 (1600ng), Asn d3,15N2(3200ng), Gly-d2 (16000ng), Gln-13C5 (3200ng), Pro-d7 (800ng), Cit-d4 (80ng), His 13C6 (400ng), Arg-13C6 (1600ng), Orn-13C5 (400ng), Lys-d8 (400ng), and pyruvic acid-13C5 (80 ng) as the internal standards for Try, Phe, Tyr, Leu, Ile, Met, Val, Glu, Thr, Ser, Asp, Ala, Asn, Gly, Gln, Pro, Cit, His, Arg, Orn, Lys, and pyruvic acid (Pyr) respectively

M., Amaral M

Tamaki, M

doi: 10.1016/j.psc.2012.12.005, 5

E., Ediger, T

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