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DTT, also known as Clelands Reagent, is an unusually strong reducing agent, owing to its high conformational proprensity to form a six member ring with an internal disulfide bond. DTT is frequently used to reduce the dissulfide bonds of proteins and, more generally, to preventintramolecularand intermoleculardisulfide bonds from forming between cysteine residues of proteins

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The adverse effects of OS on sperm quality and functions have been well documented

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