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The transfection protocol involved seeding NPCs in 6-well plates to 6070% confluence, then incubating the cells for 6 h with a transfection complex comprising 50 nM siRNA, Opti-MEM, and Lipofectamine 2,000 followed by replacing the medium with growth medium and culturing for 72 h

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Urgent medical attention is required if you develop: Severe weakness or difficulty walking Significant confusion or cognitive decline Visual disturbances Loss of bladder or bowel control For these severe neurological symptoms, seek urgent care via NHS 111, your local Emergency Department, or call 999 if severely unwell

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