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Then 3 mL culture medium made of Dulbeccos modified Eagles medium with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin was added to each well and maintained at 37 C in a humidified atmosphere of 95% air and 5% CO 2

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Methods 182:41-50 (1995)

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Tucker SL, Liao Z, Dinh J, Bian SX, Mohan R , Martel MK, Grosshans DR