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Always stick to labeled amounts

At organism-interface sites, extracellular and membrane-proximal ROS shape host defense and vascular tone: in professional phagocytes, NOX2 assembles at the phagosomal membrane to generate large bursts of O 2 into the lumen, where rapid dismutation and the action of myeloperoxidase convert upstream oxidants into hypochlorous acid (HOCl), a potent microbicidal species that collaborates with proteases and pH control to sterilize engulfed targets (Nauseef 2019)

K., DeBose-Boyd R

Therefore future efforts in neuroprotective strategies should emphasize the mitochondrial protection in astrocytes

Transpl Immunol (2015) 32(1):4650

The covalent bond formed between the Halo-tags and the linker is highly specific, irreversible, stable and occurs rapidly under physiological conditions, which facilitates the attachment to a variety of molecules or solid surfaces containing diverse functional groups (Los and Wood, 2006, Los et al., 2008, Ohana et al., 2009)