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For the assessment of mitochondrial glutathione redox potential, the MB -neuron specific GAL4 driver 201Y-GAL4 was used to express UAS-mito-roGFP2-Grx1 in WT and Cdk5-KO conditions

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Differentiated cells express key hepatocyte markersincluding albumin, tyrosine aminotransferase (TAT), and cytokeratin-18 (CK18)at both the mRNA and protein levels and exhibit essential hepatocyte functions, such as albumin secretion, glycogen storage, and cytochrome P450 7A1 expression, thereby effectively mimicking the metabolic and detoxification functions of mature hepatocytes (Malik et al., 2018)