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After washing the cells 3 times with PBS, 100 l of 1X Apollo reaction cocktail was added to each well at room temperature

PloS One 12, 124

Neuroprotective effects of a novel poly(ADP-ribose) polymerase-1 inhibitor, 2-[3-[4-(4-chlorophenyl)-1-piperazinyl] propyl]-4(3H)-quinazolinone (FR255595), in an in vitro model of cell death and in mouse 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinsons disease

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