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The iron is coordinated by four nitrogen atoms from the protoporphyrin ring

Alkaloids like caffeine contain electron-rich heterocyclic nitrogen and carbonyl groups that may participate in metal ion reduction, whereas polysaccharides and saponins provide multiple hydroxyl groups that serve as capping agents to prevent nanoparticle aggregation 27

Logistic regression analysis was used to explore the reasons for the inconsistency

the Canada Foundation for Innovation (CFI MSIF 42495)

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Various investigations have found that polymeric NPs could be a potential gene delivery system