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Synthesized RNAs were purified using MicroSpin G-25 Columns (GE Healthcare Life Sciences), quantified by Nanodrop (Invitrogen), and verified on a 5% TBE urea gel stained with SYBR Gold (Life Technologies) for 20 min prior to imaging using the ChemiDoc Imaging system (Bio-Rad Laboratories)

V., & Riul, T

10.1158/0008-5472.CAN-18-2861 142 WonE

Okra polysaccharides have been found to be effective in regulating gut microbiota, thereby controlling DM and CD

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The protocol was followed according to the manufacturers instructions with a modification, where, from day 26 on, neural organoids were placed on an orbital shake shaker (66 rpm) in a 37 C incubator during culturing