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NAD+ NAD+ (nicotinamide adenine dinucleotide) is a coenzyme that plays a critical role in cellular metabolism, DNA repair, and anti-aging processes

This reflects a very different regulatory dynamic

nitrogen dioxide (NO 2 )

Mass spectrometry was performed in dual ESI/ESI mode, with a capillary voltage of 3.0 kV, cone gas flow 50 L/h, nebulizer pressure 310 kPa, cone voltage 40 V, collision energy 2050 eV, collision-induced dissociation voltage 6 kV, desolvation gas temperature 325 C, source temperature 120 C, desolvation gas flow 600 L/h, and mass range 501500 m/z

NNMT depletes the NAD+ precursor pool by methylating nicotinamide the same nicotinamide that the salvage pathway would otherwise convert back into NAD+ via the enzyme NAMPT

Converted data were then imported to MATLAB computer and visualization environment (Release 2021b, The Mathworks, Inc., Natick, MA, USA) and analyzed with MSroi ( 45 ) chemometrics strategy using the MATLAB MSroi app ( 45 )