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Lipidomics analysis by LCMS HEK 293T cells expressing the indicated constructs were illuminated for 30 min with intermittent blue light (470 nm, 5-s pulses every min) and rinsed once in PBS

Although using fixedmetabolically inactive 148 bacteria would be helpful for dietary MUFA supplementation, many experiments using MUFAs are also RNAi experiments, which require live bacteria