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Cochleae were decalcified in 120 mM EDTA (changed daily) for 3 weeks at room temperature, with constant stirring and followed by microdissection to isolate different turns for immunolabeling

This breaks down how long a typical GHK-Cu cycle runs and what research suggests happens at each stage

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They can't

Hydrogen Sulfide and Cell Signaling

The combination consistently produces 3-8x baseline GH elevation in published models
