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Due to the slow-binding nature of UNC10201652, the IC 50 was determined as the inhibitor concentration that yielded a 50% reduction in the maximum absorbance of the uninhibited reaction, where percent inhibition was calculated as: where A exp is the end point absorbance at a particular inhibitor concentration, A max is the absorbance of the uninhibited reaction, and A bg is the background absorbance the assay