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For experiments spanning longer durations, it is best practice to aliquot the reconstituted solution into smaller, single-use volumes and store them frozen at -20C

Due to the fact that no upper limit has been determined for B12, we supplemented a megadose of B12 to mice in drinking water and challenged with Citrobacter rodentium , a natural mouse-specific pathogen that mirrors the attaching and effacing pathology seen in human EHEC infections [16]

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Liu T-C, Gurram B, Baldridge MT et al (2016) Paneth cell defects in Crohns disease patients promote dysbiosis

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Peptides are short strings of amino acids