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RSPO3, PGE2, Wnt surrogate, and FBS were added after thawing the 2 media and diluting to 1 concentration with Advanced DMEM/F12, after which the complete medium was stored at 4 C and used within one month

However, compelling cellular and ex vivo tissue models have indicated a causal role for oxidative stress (OS) in the development of IR [4][11]

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Antizyme Regulation of ODC Activity The stability of the ODC1 protein also contributes to the level of enzyme activity in cells