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Schedule uncertainty: Senolytic dosing is normally pulsed/intermittent, so a continuous daily schedule has no validated safety basis

While glucose consumption and the generation of lactate are still observed in stored erythrocytes, the rate at which these fluxes occur is insufficient to meet the demand for ATP and DPG of refrigerator-stored RBCs [144]

The connective tissue and the gall bladder were removed, and the liver was minced into small pieces and forced gently through a 200-mm-gauge stainless steel mesh using a sterile syringe plunger and then suspended in 50 ml Roswell Park Memorial Institute (RPMI)-1640 medium containing GlutaMAX-1, 25 mM HEPES and 10% FCS (pH 7.4)

Inactivation of GPX4 leads to an accumulation of lipid peroxides, resulting in ferroptotic cell death

Explants were incubated overnight at 4C with mouse monoclonal antibody against III-tubulin (Millipore, MAB5564) diluted 1:10,000 in blocking solution

doi: 10.1016/s0022-510x(97)00146-9