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HOXA methylation in normal endometrium from premenopausal women is associated with the presence of ovarian cancer: a proof of principle study

Cellular Protein Preparation and Western Blotting Following indicated treatments, cells were washed thrice with ice-cold phosphate buffered saline (PBS) and lysed in ice-cold lysis buffer (50 mM Tris-HCl, pH 7.5, with 120 mM NaCl, 10 mM sodium fluoride, 10 mM sodium pyrophosphate, 2 mM EDTA, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride, 1% NP-40, and protease inhibitor cocktail)

Ji D, Chen Y, Bi J, Shang Q, Liu H, Wang JB, Tan L, Wang J, Chen Y, Li Q, Long Q, Song L, Jiang L, Xiao G, Yu Z, Chen L, Wang X, Chen D, Li Z, Dong Z, Yang Y

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It was observed to possibly decrease fragmentation in post-ovulatory aged oocytes and during parthenogenetic activationa process where an egg develops into an embryo without fertilization

Indeed, other studies have reported the neuroprotective effect of different extracts of B