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10.3389/fphys.2017.00428 187 SeifriedH

A potential link between oxidative stress and endothelial-to-mesenchymal transition in systemic sclerosis

A study using a three-dimensional tumour sphere model combined with CRISPR-Cas9 screening reported that Nrf2 overactivation was essential for lung tumour proliferation and survival, whereas silencing of Nrf2 predisposed spherical cells to ferroptosis, which was also consistent with clinical observations [77]

Furthermore, while both reviews have discussed EVs as biomarkers, the current review provides a more detailed quantitative analysis of miRNAs in various biological fluids, offering deeper insights into their diagnostic potential

doi: 10.1016/S0925-4439(01)00025-4 5 CarrellRW

For the luciferase assay, HEK293T cells were seeded in 24-well plates until they reached 70% confluency and, subsequently, allocated into five groups: (1) transfected with GL-vector and Flag, (2) transfected with GL-VEGFA-WT and Flag-STAT6, (3) transfected with GL-VEGFA-Mut and Flag-STAT6, (4) transfected with GL-IL8-WT and Flag-STAT6, and (5) transfected with GL-IL8-Mut and Flag-STAT6
