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rAA is measured in one aliquot of a sample and total AA (that is, rAA and DHA) in a second aliquot of the same sample in which the DHA is reduced by incubation with tris(2-carboxyethyl) phosphine hydrochloride (TCEP, 0.5 mM) in the dark for 30 min at room temperature

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Double emulsion solvent evaporation techniques used for drug encapsulation