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MDA is a lipid peroxidation product, and its level can reflect the extent of oxidative stress-associated injury caused by free radicals (21)

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At last, TBARS levels were obtained by subtracting 20% of the absorbance at 453 nm from the absorbance at 532 nm (Onda Spectrophotometer, UV-21)

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All steps were performed on this staining platform by using validated reagents, including deparaffinization, antigen retrieval (cell conditioning), and Ab incubation and detection