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Mandal P, Pritchard MT, Nagy LE

EWAS were considered as inconsistent if they lacked a replication or validation cohort [24], while targeted studies were downgraded for this category if their results were in contradiction with the literature

After the membrane was blocked with 5% skim milk TBST (20 mM Tris-Cl (pH 7.4), 150 mM NaCl, 0.1% Tween-20), it was incubated with streptavidin (SA) coupled to horseradish peroxidase (HRP) (1:1000, Pierce) and washed three times with TBST for 10 min

nor the F.D.A

Luo et al., 2021), a probabilistic quotient normalization ( Despite the impact that normalization may have in correcting the noise from external variating factors, most reviewed results did not include a normalization step in their data processing pipeline (Wang et al., 2020