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Investigations of molecular changes during the honey bee mating process have primarily focused on the transcriptional profiles of the brains, ovaries, and the fat bodies of queens that are instrumentally inseminated, naturally-mated, exposed to carbon dioxide (CO 2 ), or both physically manipulated and exposed to CO 2 as compared to virgin queens 37,38,39,40,41,42

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Additionally, copper metabolism is closely linked with other trace elements

Neural differentiation has been previously described in detail, see Vermilyea et al

All pneumoconiosis patients were diagnosed in accordance with the Chinese National Diagnostic Criteria for Pneumoconiosis (GBZ70-2015), consistent with the classification of pneumoconiosis by the International Labor Organization (ILO) in 1980