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Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders (BenchMark, Invitrogen, and Kaleidoscope, BioRad)

Blood samples for pharmacokinetic analysis were collected up to 15 min pre-dose (day 1), and at 4, 8, 12, 18, 24, 30, 36, 48, 54, 60, 72, 84, 96 and 120 h post-dose

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I sometimes take MOTS-c if I remember to take it