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Lysate was centrifuged at 24,000 g for 30 min

coli demonstrate increased permeability and destabilization of the TJ proteins ZO-1, occludin, and claudin-1 [84]
doi: 10.1038/s41569-021-00569-6 90 SchlagerSGoeritzerMJandlKFreiRVujicNKolbDet al

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GHK-Cu is one of the most widely studied copper peptide compounds in skin, tissue, and extracellular matrix research

it is not a vitamin
