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After incubation for 30 min in the dark, the cells were washed with PBS three times, resuspended in 0.5 mL of PBS, and analyzed on a flow cytometer (FACSCalibur

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doi: 10.1007/s00125-002-1009-0 [DOI] [PubMed] [Google Scholar] 26.Amrein K, Scherkl M, Hoffmann M, et al

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