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For peptide materials, HPLC may support purity review, while LC-MS or mass spectrometry can support identity review [25], [26], [27]

Once activated, these microglia release ROS, inflammatory cytokines, and chemokines (Thakur et al., 2023), initiating a chronic inflammatory response and enhancing the synthesis of cathepsin B protein (Iqbal et al., 2014), intensifying neuronal cell apoptosis and contributing to AD pathogenesis