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When it quenches a free radical, it becomes oxidized and cannot quench more free radicals until it is converted back to its reduced form by special enzymes

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Neuroanatomy of Rett syndrome: a volumetric imaging study

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Afterwards aliquots of the cell suspension were treated with 10 mM DTT for 10 min at 25C and the reductant was washed out by three washing steps with HEPES buffer

Other examples of sulfhydryl-containing proteins protected by zinc are DNA zinc-binding proteins (zinc fingers), alanyl tRNA synthetase, tubulin, and dihydroorotase (Mocchegiani et al