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Similar to the published literature in the field, our co-cultured neurons show proper signs of differentiation and maturation even before 14 DIV , which is seen by many researchers as this time point in vitro (Nwabuisi-Heath et al., 2012

Ren X, Li Y, Zhou Y, Hu W, Yang C, Jing Q, et al

Cyclic AMP-specific PDE4 phosphodiesterases as critical components of cyclic AMP signaling

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According to Lee and colleagues, dietary restriction for 4 weeks in rodents resulted in increased BrdU- and NeuN-positive cells in the dentate gyrus, which indicates an increase in proliferation and neuronal differentiation, respectively [176, 177]

Type 2 diabetes and risk of hip fractures and nonskeletal fall injuries in the elderly: a study from the fractures and fall injuries in the elderly cohort (frailco)