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After exposure, zebrafish were harvested, and RNA quantified

5,10-MTHF reductase (5,10-MTHFR) carries out a central reaction in folate metabolism with the production of 5-MTHF, which is required for the biosynthesis of methionine, purine nucleotides, and thymidylate [17]

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Another aspect of advanced neuronal degeneration is represented by ghost neurons, found in the ischemic core and in the ischemic penumbra zone, which exhibits an irregular and very ill-defined cell border, pale staining cytoplasm in H&E staining and pyknotic, dark nucleus