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Slides were treated with HIER buffer (10 mM sodium citrate, 0.05% Tween-20, pH 6.0) for antigen retrieval in a pressure cooker for 10 min then sections were blocked in CAS-block histochemical reagent (Thermo Fisher Scientific, 008120)

Sun X, Zhang G, Du R, Xu R, Zhu D, Qian J, et al

S., Liu X., et al., Asparagine Endopeptidase Cleaves synuclein and Mediates Pathologic Activities in Parkinson's disease, Nature structural & molecular biology 24, no

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(2016) Natural products for the prevention of oxidative stress-related diseases: mechanisms and strategies