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Receptor knockdown was confirmed by transfecting HEK cells with mRFP-p-actin (untransfected), a 6Myc-tagged cMet gene product that served to verify presence of protein, shRNA (c-Met) sequences (only shl was employed for the knock-down experiment) and both shRNA's combined

However, the parenteral route of administration has several drawbacks, since it is invasive, painful, and poorly accepted by patients, especially when prolonged or repeated use is required [5]

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J Clin Endocrinol Metab 87 : 19891995 Johannsson G et al

The solvent was added to both the sample and standard solutions, and the mixtures were centrifuged at 13,680 g at 4 C for 10 minutes to obtain the supernatant for subsequent LC-MS/MS analysis

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