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Human adult dermal fibroblasts incubated with GHK-Cu at concentrations of 0.01, 1, and 100 nM demonstrated increased production of both elastin and collagen, with all concentrations increasing TIMP1 expression and low concentrations enhancing MMP1 and MMP2 gene expression

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This interaction may explain BPC-157's cardiovascular protective effects and its ability to enhance healing in tissues dependent on adequate blood supply

Primers used for quantitative PCR (Additional file 1 Table S1) were previously validated [29, 31, 32]

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