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25(OH)VD, insulin, glucose, GSH, protein carbonyl, and Malondialdehyde (MDA) assays Plasma levels of 25(OH)vitamin D were determined using an ELISA kit (Calbiotech, Spring Valley, CA), levels of insulin were determined using ELISA kits from ALPCO Diagnostics (Salem, NH)

(3) Glutathione peroxidase (GSH-Px, JL21016-96T, JONLNBIO) 30

Over-expression of metallothionein predicts chemoresistance in breast cancer

However, these approaches may increase ROS levels, leading to the accumulation of senescent cells [245], which can be detrimental to the patient

Whether observed changes in the microbiota are a cause or a consequence of these diseases remains a controversial issue

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