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In vitro succinylation assay Purified GCLC proteins were incubated with different concentrations of succinyl-CoA (S1129, Sigma-Aldrich) in TBS buffer (50 mM Tris-HCl [pH 7.5], 150 mM NaCl) at 37 C for 4 h

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Nevertheless, the detailed interaction pattern between the -subunit of HGF and c-MET remains unknown

Accumulation of ethanol-associated reactive molecules, e.g., reactive oxygen (ROS) and nitrogen species (RNS), and electrophilic products, e.g., acetaldehyde and lipid peroxidation-derived products, can be harmful to biological systems due to their propensity to inactivate enzymes, leading to loss of protein function, DNA damage and cell death 4

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