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The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 m 2 cm, nano Viper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 m inner diameter, 3 m resin) in buffer A (0.1%formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology

However, in some studies, no significant association was found between UPF and depression (60, 63, 64)

Roos PJ, Glerum JH, Schroeders MJ
Oncotarget 8 (18), 3060630616

doi: 10.1016/j.vascn.2010.10.004